Molecular biological marker BCL-2: testis analysis in prenatal injection of estrogen to white laboratory mice

Immunohistochemical study is one of the modern methods disease diagnostics used in practical veterinary practice, as well scientific developments differential animal diseases tumour and non-tumour nature. Prenatal influence estrogens results reproductive system disorders an adult organism which accompanied by parallel growth steroid dependent cancers offspring: testicles ovaries. The aim was to perform immunohistochemical analysis Bcl-2 marker during prenatal exposure different doses synthetic estrogen analogue Sinestrol testes offspring white non-pedigreed laboratory mice. After fertilization, pregnant females were divided into 3 groups, intact two experimental groups. group unaffected (n = 10). first group, C-25 13), injected with drug form a 2 % oil solution at dose 25 g/kg. second 13) given preparation 40 mkg/kg. When reached sexual maturity, they removed from experiment. carried out on sections paraffin blocks progeny intended for standard morphological study, apoptosis inhibitor determined indices cellular elements male glands progeny: spermatogonia, spermatocytes, sperm-tides, spermatozoa Leydig cells. Expression upon g/kg showed that number positively stained cells spermatogonia increased 8.6 9.4 respectively compared group. groups C-40, expression spermatocyte no difference, slight increase spermatids observed. rate C C-40 decreased 56.0 (P 0.05) 60.0 0.05), respectively. Administration fetal gland initiation resulted impaired morphology adulthood. index cells, resulting apoptotic cell death, responsible production sex hormone testosterone. can be select optimal period.